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KMID : 0613820100200111656
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Journal of Life Science
2010 Volume.20 No. 11 p.1656 ~ p.1661
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Hydrolysis of Egg Yolk Protein in a Packed Bed Reactor by Immobilized Enzyme
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Kang Byung-Chul
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Abstract
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Alkaline protease for the hydrolysis of egg yolk protein was immobilized on five carriers ? Duolite A568, Celite R640, Dowex-1, Dowex 50W and Silica gel R60. Duolite A568 showed a maximum immobilization yield of 24.7%. Optimum pH for the free and immobilized enzyme was pH 8 and 9, respectively. However, no change was observed in optimum temperature (50℃). Thermal stability was observed in immobilized enzymes compared to free enzymes. The immobilized enzyme retained 86% activity after 10 cycle operations in a repeated batch process. The effect of flow rate on the stability of enzyme activity in continuous packed-bed reactor was investigated. Lowering flow rate increased the stability of the immobilized enzyme. After 96 hr of continuous operation in a packed-bed reactor, the immobilized enzyme retained 83 and 61% activity when casein and egg yolk were used as a raw materials, respectively.
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KEYWORD
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Enzyme immobilization, egg yolk protein, packed-bed reactor, continuous operation, repeated batch
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